Method SM 5520E

Oil & Grease - Extraction Method for Sludge Samples

Oil and grease is composed primarily of fatty matter from animal and vegetable sources and from hydrocarbons of petroleum origin. The portion of oil and grease from each of these two major sources can be determined with Method 5520F. A knowledge of the relative composition of a sample minimizes the difficulty in determining the majorsource of the material and simplifies the correction of oil and grease problems in wastewater treatment plant operation and stream pollution abatement. Method E is a modification of the Soxhlet method and is suitable for sludges and similar materials. Drying acidified sludge by heating leads to low results. Magnesium sulfate monohydrate is capable of combining with 75% of its own weight in water in forming magnesium sulfate heptahydrate, and is used to dry sludge. After drying, the oil and grease can be extracted with an organic solvent. Method F can be used in conjunction with Method E to obtain a hydrocarbon measurement in addition to, or instead of, the oil and grease measurement. This method makes use of silica gel to separate hydrocarbons from the total oil and grease on the basis of polarity.

(Standard Methods)


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Method Data

Hold Times, Preservatives, Preps, Collection, Analytical & Documentation
Holding Time:   Analyze as soon as possible.
Preservatives:   If analysis is to be delayed for more than 2 hours, acidify to pH 2 or lower with either 1:1 HCl or 1:1 sulfuric acid and refrigerate.
Required Preps:   Wide mouth glass bottle
Collection Method:   Grab Sampling
Analytical Methodology:   Extraction

Analyte List*

Analyte Formula CAS Number Detection Limit
Oil & Grease

* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.