This method is for the collection and analysis of pentamidine isethionate from workplace air. The sample is collected on a PVC filter. After collection the filter is eluted with 3mL of 50:50 ethanol:water containing 0.085% H3PO4 and 0.04% TMAC and analzyed by HPLC with UV detection.
The working range of the method is 0.5 to 30 ug/m3 for a 100L air sample.
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| Hold Times, Preservatives, Preps, Collection, Analytical & Documentation | |
|---|---|
| Holding Time: | The method says the sample is stable for greater than 27 days if kept in the dark at 25°C. |
| Preservatives: | Keep samples in the dark at 25°C from collection to analysis. |
| Required Preps: | 37mm PVC filter |
| Collection Method: | The method recommends a 1 - 2 L/min. flow rate with a 50 - 1500L sample collection volume. |
| Analytical Methodology: | HPLC/UV |
| Documentation: | 
5032
|
| Analyte | Formula | CAS Number | Detection Limit | |
|---|---|---|---|---|
| Pentamidine isethionate | C19H24N4O2.2C2H6O4S |
140-64-7 |
18 |
ng |
* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.