Method 5031

Aspartame by HPLC-UV


This method is for the determination of aspartame. The sample is collected on PTFE membrane filters, extracted with 2mL of eluent B, followed by HPLC analysis with ultraviolet detection. The working range is 0.07 to 8 mg/m³ for a 100-L air sample. The method has been used to analyze personal and area air samples during a health hazard evaluation at a commercial food packaging plant.


(NIOSH Issue 2: 15 August 1994)

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Method Data

Hold Times, Preservatives, Preps, Collection, Analytical & Documentation
Holding Time:   Stable for at least 30 days.
Preservatives:   Store at room temperature.
Required Preps:   PTFE membrane filter: 1-µm pore size, 37-mm diameter.
Collection Method:   PTFE filter: 70L - 1200L @ 1-3L/min.
Analytical Methodology:   HPLC/UV
Documentation:   5031

Analyte List*

Analyte Formula CAS Number Detection Limit
Aspartame
22839-47-0
2
 µg

* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.