This method is for the determination of benzidine-, o-tolidine-, and o-dianisidine- dyes. The sample is collected on PTFE filters, desorbed with 2mL water in an ultrasonic bath, followed by HPLC analysis with ultraviolet detection. The working range is about 0.06 to 8 mg/m³ for a 250-L air sample. This method determines benzidine, o-tolidine or o-dianisidine from dyes based on these amines, but will not distinguish different dyes based on the same amine. The method will not distinguish benzidine obtained from reduction of the dyes and free benzidine in the dye formulation. Note: This revises P&CAM 325.
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| Hold Times, Preservatives, Preps, Collection, Analytical & Documentation | |
|---|---|
| Holding Time: | At least 7 days @ 25 °C in the dark. |
| Preservatives: | Keep samples dry and cool; protect from light during shipping and storage. |
| Required Preps: | 5-µm PTFE membrane filter. |
| Collection Method: | PTFE membrane filter: 150L - 500L @ 1-3L/min. |
| Analytical Methodology: | HPLC/UV |
| Documentation: | 
5013
|
| Analyte | Formula | CAS Number | Detection Limit | |
|---|---|---|---|---|
| C.I. Direct Brown 95 | 16071-86-6 |
3 |
µg | |
| C.I. Direct Black 38 | 1937-37-7 |
3 |
µg | |
| C.I. DIRECT BLUE 8 | 2429-71-2 |
3 |
µg | |
| C.I. DIRECT BLUE 6 | 2602-46-2 |
3 |
µg | |
| C.I. Direct Red 28 | 573-58-0 |
3 |
µg | |
| C.I. Direct Red 2 | 992-59-6 |
3 |
µg | |
* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.