This method is for the determination of rotenone. The sample is collected on 1-µm PTFE membrane filters with backup pads and extracted in 4mL acetonitrile. The working range is 0.4 to 10 mg/m³ for a 100-L air sample and the method is applicable to commercial formulations. Rotenone, a naturally occurring insecticide, is adequately separated by HPLC from other compounds (e.g., sumatrol, a-toxicarol, deguelin, elliptone, malaccol, and tephrosin) present in commercial cube root extracts. Rotenone is sensitive to photodecomposition.
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| Hold Times, Preservatives, Preps, Collection, Analytical & Documentation | |
|---|---|
| Holding Time: | At least 7 days @ 25 °C in dark |
| Preservatives: | Keep from direct exposure to light. |
| Required Preps: | 1-µm PTFE membrane filter with backup pad. |
| Collection Method: | 1-µm PTFE membrane filter; 8L to 400L @ 1-4L/min. |
| Analytical Methodology: | HPLC/UV |
| Documentation: | 
5007
|
| Analyte | Formula | CAS Number | Detection Limit | |
|---|---|---|---|---|
| Rotenone | C23H22O6 |
83-79-4 |
4 |
µg |
* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.