This method is for the determination of nicotine. Samples are collected on XAD-4 sorbent tubes and desorbed in 1 mL ethyl acetate with 0.01% triethylamine. The sample is then analyzed by Gas Chromatography with a nitrogen selective detector. Under the GC parameters stated in the method, nicotine can be identified based upon retention time. Nicotine is quantified using quinoline as an internal standard. At high sample concentration, XAD-4 sorbent tube capacity (300 µg) may be exceeded and breakthrough may occur. Other methods for nicotine are NMAM 2544 (August 15,1994) and Method S293 on which it was based. This method replaces the XAD-2 sorbent with XAD-4, and uses a capillary column in place of a packed GC column. The ethyl acetate desorption solvent was modified to improve nicotine recovery from sides of glass sorbent tube, and to improve recoveries for the lower DE levels.
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| Hold Times, Preservatives, Preps, Collection, Analytical & Documentation | |
|---|---|
| Holding Time: | 14 days at 5°C in dark. |
| Preservatives: | Keep refrigerated and from prolonged light exposure. |
| Required Preps: | Solid sorbent tube; XAD-4 80mg/40mg; 7cm x 7mm OD. |
| Collection Method: | Solid sorbent tube 0.5 L to 600 L @ 0.1 to 1 L/min. |
| Analytical Methodology: | GC/NPD |
| Documentation: | 
2551
|
| Analyte | Formula | CAS Number | Detection Limit | |
|---|---|---|---|---|
| Nicotine | C10H14N2 |
54-11-5 |
0.013 |
µg |
* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.