Method 974.29

Vitamin A in Mixed Feeds, Premixes, and Human and Pet Foods

This method is for the determination of Vitamin A in mixed feeds, premixes, human and pet foods. Dry mixed feeds, premixes, food should be ground so that more than 95% of the sample passes through a No. 20 sieve. The sample is hydrolized with a KOH solution and analyzed by spectophotometry.

This method is not applicable to products which contain provitamin A (carotene) as a predominant source of vitamin A activity nor to high potency vitamin A concentrates used for feed, premix, and food manufacture.



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Method Data

Hold Times, Preservatives, Preps, Collection, Analytical & Documentation
Holding Time:   Do not store ground test samples for more than 1 week.
Preservatives:   Dry mixes and premixes: keep refrigerated away from light in a tightly closed glass or plastic container. Liquid feed supplements and premixes: Store in a cool place ≥ 8 °C in a tightly closed glass or plastic container.
Required Preps:   500 g Amber glass or plastic bottle.
Collection Method:   Grab sampling
Analytical Methodology:   Colorimeter
Documentation:   974.29

Analyte List*

Analyte Formula CAS Number Detection Limit
Vitamin A
 IU/100 g

* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.