This method is for the determination of quinic, malic and citric acid from cranberry juice cocktail and apple juice. The sample. The sample is extraction using a solid-phase extraction. The juice is diluted with water and passed through a disposable cartidge (Sep Pak C18 or equivalent), filtered and analyzed by high performance liquid chromatography (HPLC) with UV detection.
Note that many labs modify the method to analyze for other organic acids such as acetic, fumaric, glutaric, lactic, oxalic, suucinic, and tartaric.
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| Hold Times, Preservatives, Preps, Collection, Analytical & Documentation | |
|---|---|
| Holding Time: | None specified in the method. |
| Preservatives: | None specified in the method. |
| Required Preps: | 125mL Glass bottle with Teflon-lined lid |
| Collection Method: | Grab sampling |
| Analytical Methodology: | HPLC |
| Documentation: | |
| Analyte | Formula | CAS Number | Detection Limit | |
|---|---|---|---|---|
| Malic acid | C4H6O5 |
6915-15-7 |
100 |
ppm |
| Citric acid | C6H8O7 |
77-92-9 |
100 |
ppm |
| Quinic acid | C7H12O6 |
77-95-2 |
100 |
ppm |
* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.