This method is for the determination of phospholipids from soy lecithin. The sample is diluted with chloroform:methanol and analyzed by high performance chromatography with evaporative light scattering detector (HPLC/ELSD).
Phospholipids are extremely temperature sensitive and the sample should be kept frozen from the time of collection to analysis.
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| Hold Times, Preservatives, Preps, Collection, Analytical & Documentation | |
|---|---|
| Holding Time: | None specified in the method. |
| Preservatives: | Keep samples frozen from sample collection to analysis. |
| Required Preps: | 10g Glass or plastic bottle with Teflon-lined lid |
| Collection Method: | Grab sampling |
| Analytical Methodology: | HPLC/ELSD |
| Documentation: | |
| Analyte | Formula | CAS Number | Detection Limit | |
|---|---|---|---|---|
| Phosphotidylchloline (PC) | 0.625 |
weight % | ||
| Phosphotidylethanolamine (PE) | 0.625 |
weight % | ||
| Phosphatidic acid (PA) | 0.625 |
weight % | ||
| Phosphatidylinositol (PI) | 0.625 |
weight % | ||
| Phosphatidylserine (PS) | 0.625 |
weight % | ||
| Lisophospholipid (LPC) | 0.625 |
weight % | ||
* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.