This method is for the determination of amino acid from food and food ingredients. Three aliquots of the sample are taken and prepared using acid hydrolysis, acid oxidation followed by acid hydrolysis, and alkaline hydrolysis. All three extracts from the procedures are then analyzed by an amino acid analyzer. Each hydrolysate is used to determine different amino acids.
The percentage recovery of each amino acid is computed determining the nitrogen content for each amino acid.
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| Hold Times, Preservatives, Preps, Collection, Analytical & Documentation | |
|---|---|
| Holding Time: | None specified in the method. |
| Preservatives: | None specified in the method. |
| Required Preps: | None |
| Collection Method: | Grab sampling |
| Analytical Methodology: | Amino acid analyzer |
| Documentation: | 
982.30
|
| Analyte | Formula | CAS Number | Detection Limit | |
|---|---|---|---|---|
| Proline | C5H9NO2 |
147-85-3 |
0.01 |
% |
| Glycine | C2H5NO2 |
56-40-6 |
0.01 |
% |
| Alanine | C3H7NO2 |
56-41-7 |
0.01 |
% |
| L-Serine | C3H7NO3 |
56-45-1 |
0.01 |
% |
| Aspartic acid | C4H7NO4 |
56-84-8 |
0.01 |
% |
| L-Glutamic acid | C5H9NO4 |
56-86-0 |
0.01 |
% |
| Lysine | C6H14N2O2 |
56-87-1 |
0.01 |
% |
| Tyrosine | C9H11NO3 |
60-18-4 |
0.01 |
% |
| Leucine | C6H13NO2 |
61-90-5 |
0.01 |
% |
| L-Methionine | C5H11NO2S |
63-68-3 |
0.01 |
% |
| L-Phenylalanine | C9H11NO2 |
63-91-2 |
0.01 |
% |
| L-Histidine | C6H9N3O2 |
71-00-1 |
0.01 |
% |
| Valine | C5H11NO2 |
72-18-4 |
0.01 |
% |
| Threonine | C4H9NO3 |
72-19-5 |
0.01 |
% |
| Isoleucine | C6H13NO2 |
73-32-5 |
0.01 |
% |
| L-Arginine | C6H14N4O2 |
74-79-3 |
0.01 |
% |
| Cystine | C6H12N2O4S2 |
24645-67-8 |
0.01 |
% |
* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.