This is a flame ionization detector gas chromatographic (FIDGC) method applicable to the determination of phenol and certain substituted phenols in municipal and industrial discharges as provided under 40 CFR Part 136.1. A measured volume of sample, approximately 1 L, is acidified and extracted with methylene chloride using a separatory funnel. The methylene chloride extract is dried and exchanged to 2-propanol during concentration to a volume of 10 mL or less. The extract is separated by gas chromatography and the phenols are then measured with an FID. A preliminary sample wash under basic conditions can be employed for samples having high general organic and organic base interferences. The method also provides for a derivatization and column chromatography cleanup procedure to aid in the elimination of interferences. The derivatives are analyzed by ECDGC.
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| Hold Times, Preservatives, Preps, Collection, Analytical & Documentation | |
|---|---|
| Holding Time: | All samples must be extracted within seven days of collection and completely analyzed within 40 days of extraction. |
| Preservatives: | All samples must be iced or refrigerated at 4°C from the time of collection until extraction. If residual chlorine is present, add 80 mg of sodium thiosulfate per liter of sample and mix well. |
| Required Preps: | Glass bottle |
| Collection Method: | Grab or composite sampling |
| Analytical Methodology: | GC/FID |
| Documentation: | 
604
|
| Analyte | Formula | CAS Number | Detection Limit | |
|---|---|---|---|---|
| 4-Nitrophenol | C6H5NO3 |
100-02-7 |
2.8 |
µg/L |
| 2,4-Dimethylphenol | C8H10O |
105-67-9 |
0.32 |
µg/L |
| Phenol | C6H6O |
108-95-2 |
0.14 |
µg/L |
| 2,4-Dichlorophenol | C6H4Cl2O |
120-83-2 |
0.39 |
µg/L |
| 2,4-Dinitrophenol | C6H4N2O5 |
51-28-5 |
13 |
µg/L |
| 4,6-Dinitro-2-methyl phenol | C7H6N2O5 |
534-52-1 |
16 |
µg/L |
| 4-Chloro-3-methylphenol | C7H7ClO |
59-50-7 |
0.36 |
µg/L |
| Pentachlorophenol | C6HCl5O |
87-86-5 |
7.4 |
µg/L |
| 2,4,6-Trichlorophenol | C6H3Cl3O |
88-06-2 |
0.64 |
µg/L |
| 2-Nitrophenol | C6H5NO3 |
88-75-5 |
0.45 |
µg/L |
| 2-Chlorophenol | C6H5ClO |
95-57-8 |
0.31 |
µg/L |
* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.