This method describes a procedure for determination of certain polycyclic aromatic hydrocarbons (PAH) in drinking water sources and finished drinking water. A measured volume of sample, approximately 1 L, is serially extracted with methylene chloride using a separatory funnel. The methylene chloride extract is dried and concentrated to a volume of 1 mL. A 3.0 mL portion of acetonitrile is added to the extract and concentrated to a final volume of 0.5 mL. The extract analytes are then separated by HPLC. Ultraviolet adsorption (UV) and fluorescence detectors are used with HPLC to quantitatively measure the PAHs.
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| Hold Times, Preservatives, Preps, Collection, Analytical & Documentation | |
|---|---|
| Holding Time: | All samples must be extracted within seven days of collection and completely analyzed within 30 days of extraction. |
| Preservatives: | All samples must be iced at 4°C from the time of collection until extraction; stored in amber or foil-wrapped bottles in order to minimize photolytic decomposition; acidified to pH<2 with HCl to inhibit biological activity. In the presence of residual chlorine, sodium thiosulfate is added. |
| Required Preps: | Glass bottle |
| Collection Method: | Grab or composite sampling |
| Analytical Methodology: | HPLC with Coupled Ultraviolet and Fluorescence Detection. |
| Documentation: | 
550
|
| Analyte | Formula | CAS Number | Detection Limit | |
|---|---|---|---|---|
| Anthracene | C14H10 |
120-12-7 |
0.079 |
µg/L |
| Pyrene | C16H10 |
129-00-0 |
0.126 |
µg/L |
| Benzo(g,h,i)perylene | C22H12 |
191-24-2 |
0.014 |
µg/L |
| Indeno(1,2,3-cd)pyrene | C22H12 |
193-39-5 |
0.011 |
µg/L |
| Benzo[b]fluoranthene | C20H12 |
205-99-2 |
0.003 |
µg/L |
| Fluoranthene | C16H10 |
206-44-0 |
0.026 |
µg/L |
| Benzo[k]fluoranthene | C20H12 |
207-08-9 |
0.002 |
µg/L |
| Acenaphthylene | C12H8 |
208-96-8 |
2.3 |
µg/L |
| Chrysene | C18H12 |
218-01-9 |
0.063 |
µg/L |
| Benzo[a]pyrene | C20H12 |
50-32-8 |
0.029 |
µg/L |
| Dibenz[a,h]anthracene | C22H14 |
53-70-3 |
0.019 |
µg/L |
| Benzo(a)anthracene | C18H12 |
56-55-3 |
0.002 |
µg/L |
| Acenaphthene | C12H10 |
83-32-9 |
3 |
µg/L |
| Phenanthrene | C14H10 |
85-01-8 |
0.162 |
µg/L |
| Fluorene | C13H10 |
86-73-7 |
0.25 |
µg/L |
| Naphthalene | C10H8 |
91-20-3 |
3.3 |
µg/L |
* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.