Method 550

Determination of Polycyclic Aromatic Hydrocarbons in Drinking Water by Liquid-Liquid Extraction and HPLC with Coupled Ultraviolet and Fluorescence Detection


This method describes a procedure for determination of certain polycyclic aromatic hydrocarbons (PAH) in drinking water sources and finished drinking water. A measured volume of sample, approximately 1 L, is serially extracted with methylene chloride using a separatory funnel. The methylene chloride extract is dried and concentrated to a volume of 1 mL. A 3.0 mL portion of acetonitrile is added to the extract and concentrated to a final volume of 0.5 mL. The extract analytes are then separated by HPLC. Ultraviolet adsorption (UV) and fluorescence detectors are used with HPLC to quantitatively measure the PAHs.


(EPA: Office of Water)

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Method Data

Hold Times, Preservatives, Preps, Collection, Analytical & Documentation
Holding Time:   All samples must be extracted within seven days of collection and completely analyzed within 30 days of extraction.
Preservatives:   All samples must be iced at 4°C from the time of collection until extraction; stored in amber or foil-wrapped bottles in order to minimize photolytic decomposition; acidified to pH<2 with HCl to inhibit biological activity. In the presence of residual chlorine, sodium thiosulfate is added.
Required Preps:   Glass bottle
Collection Method:   Grab or composite sampling
Analytical Methodology:   HPLC with Coupled Ultraviolet and Fluorescence Detection.
Documentation:   550

Analyte List*

Analyte Formula CAS Number Detection Limit
Anthracene
C14H10
120-12-7
0.079
 µg/L
Pyrene
C16H10
129-00-0
0.126
 µg/L
Benzo(g,h,i)perylene
C22H12
191-24-2
0.014
 µg/L
Indeno(1,2,3-cd)pyrene
C22H12
193-39-5
0.011
 µg/L
Benzo[b]fluoranthene
C20H12
205-99-2
0.003
 µg/L
Fluoranthene
C16H10
206-44-0
0.026
 µg/L
Benzo[k]fluoranthene
C20H12
207-08-9
0.002
 µg/L
Acenaphthylene
C12H8
208-96-8
2.3
 µg/L
Chrysene
C18H12
218-01-9
0.063
 µg/L
Benzo[a]pyrene
C20H12
50-32-8
0.029
 µg/L
Dibenz[a,h]anthracene
C22H14
53-70-3
0.019
 µg/L
Benzo(a)anthracene
C18H12
56-55-3
0.002
 µg/L
Acenaphthene
C12H10
83-32-9
3
 µg/L
Phenanthrene
C14H10
85-01-8
0.162
 µg/L
Fluorene
C13H10
86-73-7
0.25
 µg/L
Naphthalene
C10H8
91-20-3
3.3
 µg/L

* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.