Method 507

Determination of Nitrogen- and Phosphorous- Containing Pesticides in Water by Gas Chromatography With a Nitrogen Phosphorous Detector


This is a gas chromatographic (GC) method applicable to the determination of certain nitrogen- and phosphorus-containing pesticides in ground water and finished drinking water. A measured volume of sample of approximately 1 L is extracted with methylene chloride by shaking in a separatory funnel or mechanical tumbling in a bottle. The methylene chloride extract is isolated, dried and concentrated to a volume of 5 mL during a solvent exchange to methyl tert-butyl ether. Chromatographic conditions are described which permit the separation and measurement of the analytes in the extract by Capillary Column GC with a nitrogen-phosphorus detector (NPD).


(EPA: Office of Water)

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Method Data

Hold Times, Preservatives, Preps, Collection, Analytical & Documentation
Holding Time:   Analytes stored are stable up to 14 days; however, some analytes must be extracted immediately. A 14-day maximum extract storage time is recommended.
Preservatives:   If residual chlorine is present, add 80 mg of sodium thiosulfate per liter of sample to the sample bottle prior to collecting the sample. The samples must be iced or refrigerated at 4°C away from light from the time of collection until extraction.
Required Preps:   Glass bottle
Collection Method:   Grab sampling
Analytical Methodology:   GC/NPD (Nitrogen Phosphorous Detector)
Documentation:   507

Analyte List*

Analyte Formula CAS Number Detection Limit
Chlorpropham
C10H12ClNO2
101-21-3
0.2
 µg/L
Simetryn
C8H15N5S
1014-70-6
0.035
 µg/L
Pebulate
C10H21NOS
1114-71-2
0.022
 µg/L
Cycloate
C11H21NOS
1134-23-2
0.022
 µg/L
MGK-264
C17H25NO2
113-48-4
0.19
 µg/L
Simazine
C7H12ClN5
122-34-9
0.014
 µg/L
Terbufos
C9H21O2PS3
13071-79-9
0.054
 µg/L
Ethoprop
C8H19O2PS2
13194-48-4
0.021
 µg/L
Propazine
C9H16ClN5
139-40-2
0.014
 µg/L
Merphos
C12H27PS3
150-50-5
0.04
 µg/L
Napropamide
C17H21NO2
15299-99-7
0.069
 µg/L
Alachlor (Lasso)
C14H20ClNO2
15972-60-8
0.14
 µg/L
Atraton
C9H17N5O
1610-17-9
0.17
 µg/L
Prometon
C10H19N5O
1610-18-0
0.041
 µg/L
Atrazine
C8H14ClN5
1912-24-9
0.015
 µg/L
Vernolate
C10H21NOS
1929-77-7
0.055
 µg/L
Butylate
C11H23NOS
2008-41-5
0.053
 µg/L
Metribuzin (Sencor)
C8H14N4OS
21087-64-9
0.029
 µg/L
Molinate
C9H17NOS
2212-67-1
0.061
 µg/L
Fenamiphos (Nemacur)
C13H22NO3PS
22224-92-6
0.12
 µg/L
Tetrachlorvinphos
C10H9Cl4O4P
22248-79-9
0.18
 µg/L
Butachlor
C17H26ClNO2
23184-66-9
0.12
 µg/L
Pronamide (Kerb, Propyzamide)
C12H11Cl2NO
23950-58-5
0.28
 µg/L
Disulfoton sulfone
C8H19O4PS3
2497-06-5
0.63
 µg/L
Disulfoton sulfoxide
2497-07-6
0.082
 µg/L
Norflurazon
C12H9ClF3N3O
27314-13-2
0.098
 µg/L
Disulfoton
C8H19O2PS3
298-04-4
0.029
 µg/L
Bromacil
C9H13BrN2O2
314-40-9
0.69
 µg/L
Diazinon
C12H21N2O3PS
333-41-5
0.13
 µg/L
Tebuthiuron
C9H16N4OS
34014-18-1
0.58
 µg/L
Tricyclazole
C9H7N3S
41814-78-2
0.21
 µg/L
Triadimefon (Bayleton)
C14H16ClN3O2
43121-43-3
0.093
 µg/L
Metolachlor
C15H22ClNO2
51218-45-2
0.19
 µg/L
Hexazinone
C12H20N4O2
51235-04-2
0.15
 µg/L
Carboxin
C12H13NO2S
5234-68-4
0.18
 µg/L
Terbacil
C9H13ClN2O2
5902-51-2
0.56
 µg/L
Fluridone
C19H14F3NO
59756-60-4
2.8
 µg/L
Fenarimol
C17H12Cl2N2O
60168-88-9
0.2
 µg/L
Dichlorvos
C4H7Cl2O4P
62-73-7
0.28
 µg/L
Prometryn
C10H19N5S
7287-19-6
0.024
 µg/L
EPTC
C9H19NOS
759-94-4
0.08
 µg/L
Mevinphos (Phosdrin)
C7H13O6P
7786-34-7
0.87
 µg/L
Ametryne
C9H17N5S
834-12-8
0.2
 µg/L
Terbutryn
C10H19N5S
886-50-0
0.031
 µg/L
Methyl Paraoxon
950-35-6
0.3
 µg/L
Diphenamid
C16H17NO
957-51-7
0.082
 µg/L

* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.