Method 614

Determination of Organophosphorus Pesticides in Municipal and Industrial Wastewater


This method is for the determination of organophosphorus pesticides in municipal and industrial wastewater.  One liter of sample is extracted with 15% methylene chloride in hexane using a separatory funnel.  The extract is dried and concentrated to a volume of 10 mL or less and then analyzed using gas chromatography with a flame photometric or thermionic bead detector (GC/FPD).

The method specifies use of cleanup procedures when needed because of the sample matrix.  Acetonitrile partitioning and Florisil column cleanup are approved for use.  


(EPA: Office of Water)

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Method Data

Hold Times, Preservatives, Preps, Collection, Analytical & Documentation
Holding Time:   7 days from collection to extraction and 40 days from extraction to analysis.
Preservatives:   Cool to 4°C from the time of sample collection until extraction.
Required Preps:   1L amber glass bottle
Collection Method:   Grab sampling
Analytical Methodology:   GC/FPD
Documentation:   614

Analyte List*

Analyte Formula CAS Number Detection Limit
Malathion
C10H19O6PS2
121-75-5
 
Methyl parathion
C8H10NO5PS
298-00-0
0.012
 µg/L
Disulfoton
C8H19O2PS3
298-04-4
 
Diazinon
C12H21N2O3PS
333-41-5
0.012
 µg/L
Ethion
C9H22O4P2S4
563-12-2
 
Ethyl parathion
C10H14NO5PS
56-38-2
0.012
 µg/L
Demeton
C8H19O3PS2
8065-48-3
 
Azinphos-methyl (Guthion)
C10H12N3O3PS2
86-50-0
 

* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.