Method 413.2

Oil And Grease (Spectrophotometric, Infrared)

The definition of oil and grease is based on the procedure used. The source of the oil and/or grease, and the presence of extractable non-oily matter will influence the material measured and interpretation of results. This method includes the measurement of fluorocarbon-113 extractable matter from surface and saline waters, industrial and domestic wastes. It is applicable to the determination of hydrocarbons, vegetable oils, animal fats, waxes, soaps, greases and related matter. The method is applicable to measurement of most light petroleum fuels, although loss of about half of any gasoline present during the extraction manipulations can be expected. The method covers the range from 0.2 to 1000 mg/L of extractable material. While this method can be used to obtain an estimate of the oil and grease that would be measured gravimetrically, in many cases the estimate more accurately describes the parameter, as it will measure volatiles more effectively and is not susceptible to interferences such as extractable sulfur. It can be used with the Petroleum Hydrocarbon procedure to obtain an oil and grease value and a petroleum hydrocarbon value on the same sample. The sample is acidified to a low pH ( < 2) and extracted with fluorocarbon-113. The oil and grease is determined by comparison of the infrared absorbance of the sample extract with standards.

(EPA: Office of Water)


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Method Data

Hold Times, Preservatives, Preps, Collection, Analytical & Documentation
Holding Time:   Analyze as soon as possible.
Preservatives:   If analysis is to be delayed for more than a few hours, the sample is preserved by the addition of 5 mL HCl at the time of collection and refrigerated at 4°C.
Required Preps:   Glass bottle
Collection Method:   Grab sampling
Analytical Methodology:   Spectrophotometric

Analyte List*

Analyte Formula CAS Number Detection Limit
Oil & Grease

* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.