Method 9066

Phenolics (Colorimetric, Automated 4-AAP with Distillation)

This method is for the determination of phenolics from ground water and of drinking, surface, and saline waters.  This is an automated method based on the distillation of phenol and subsequent reaction of the distillate with alkaline ferricyanide and 4-amino-antipyrine (4-AAP) to form a red complex which is measured at colorimetrically at 505 or 520 nm.

The method is capable of measuring phenolic materials from 2 to 500 µg/L in the aqueous phase using phenol as a standard. 

Chlorine and other oxidizing agents that are detected by the liberation of iodine upon acidification in the presence of potassium iodide are removed by adding an excess of ferrous ammonium sufate immediately after sampling.  If the chlorine is not removed the phenolic compounds may be partially oxidized and the results may be low.

(EPA SW-846)


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Method Data

Hold Times, Preservatives, Preps, Collection, Analytical & Documentation
Holding Time:   Analyze within 28 days of sample collection.
Preservatives:   Acidify to a pH < 4 with phosphoric acid to inhibit biological degradation. Cool sample to 4°C.
Required Preps:   500 mL amber glass bottle
Collection Method:   Grab sampling
Analytical Methodology:   Colorimetric
Documentation:   9066

Analyte List*

Analyte Formula CAS Number Detection Limit
Total Phenol

* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.