Method 335.3

Cyanide, Total (Colorimetric, Automated UV)

This method is applicable to the determination of cyanide in drinking and surface waters, domestic and industrial wastes. The applicable range is 5 to 500 µg/L. The cyanide as hydrocyanic acid (HCN), is released from cyanide complexes by means of UV digestion and distillation. Cyanides are converted to cyanogen chloride by reactions with chloramine-T which subsequently reacts with pyridine and barbituric acid to give a red-colored complex.

(EPA: Office of Water)


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Method Data

Hold Times, Preservatives, Preps, Collection, Analytical & Documentation
Holding Time:   Samples should be analyzed as rapidly as possible after collection. If storage is required, the samples should be stored in a refrigerator or in an ice chest filled with water and ice to maintain temperature at 4°C.
Preservatives:   Samples must be preserved with 2 mL of 10 N sodium hydroxide per liter of sample (pH > or = 12) at the time of collection.
Required Preps:   None noted in method.
Collection Method:   The sample should be collected in plastic bottles of 1 liter or larger size.
Analytical Methodology:   Technicon Auto Analyzer; Manifold with UV digester; Colorimeter equipped with a 15 mm flowcell and 570 nm filter.
Documentation:   335.3

Analyte List*

Analyte Formula CAS Number Detection Limit

* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.