Method 413.1

Oil And Grease (Gravimetric, Separatory Funnel Extraction)

The definition of oil and grease is based on the procedure used. The nature of the oil and/or grease, and the presence of extractable non-oily matter will influence the material measured and interpretation of results. This method includes the measurement of fluorocarbon-113 extractable matter from surface and saline waters, industrial and domestic wastes. It is applicable to the determination of relatively non-volatile hydrocarbons, vegetable oils, animal fats, waxes, soaps, greases and related matter. The method is not applicable to measurement of light hydrocarbons that volatilize at temperatures below 70°C. Petroleum fuels from gasoline through #2 fuel oils are completely or partially lost in the solvent removal operation. Some crude oils and heavy fuel oils contain a significant percentage of residue-type materials that are not soluble in fluorocarbon-113. Accordingly, recoveries of these materials will be low. The method covers the range from 5 to 1000 mg/L of extractable material. The sample is acidified to a low pH ( < 2) and serially extracted with fluorocarbon- 113 in a separatory funnel. The solvent is evaporated from the extract and the residue weighed.

(EPA: Office of Water)


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Method Data

Hold Times, Preservatives, Preps, Collection, Analytical & Documentation
Holding Time:   Analyze as soon as possible.
Preservatives:   If analysis is to be delayed for more than a few hours, the sample is preserved by the addition of 5 mL HCl at the time of collection and refrigerated at 4°C.
Required Preps:   Glass bottle
Collection Method:   Grab sampling
Analytical Methodology:   Gravimetric
Documentation:   413.1

Analyte List*

Analyte Formula CAS Number Detection Limit
Oil & Grease

* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.