Method 410.4

The Determination of Chemical Oxygen Demand (COD) by Semi-Automated Colorimetry

This is for the determination of chemical oxygen demand (COD) from ground and surface waters, domestic and industrial wastes. The samples, blanks and standards are placed into sealed tubes and heated in an oven or block digester in the presence of dichromate at 150°C.  After 2 hours the samples are removed from the oven or digester, cooled and measured spectrophotometrically at 600 nm.  The colorimetric determination can also be determined manually.

The applicable range of the automated method is 3-900 mg/L and the range of the manual method is 20 to 900 mg/L.

Chlorides are quantitatively oxidized by dichromate and represent a positive interference.  Mercuric sulfate is added to the digestion tubes to complex the chlorides.

(EPA: Office of Water)


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Method Data

Hold Times, Preservatives, Preps, Collection, Analytical & Documentation
Holding Time:   28 days if samples are preserved samples and maintained at 4°C.
Preservatives:   Samples must be preserved with sulfuric acid to a pH <2 and cooled to 4°C as siib as possible after collection.
Required Preps:   500 mL amber glass or plastic bottle
Collection Method:   Grab sampling
Analytical Methodology:   Spectrophotometer or Technicon Analyzer
Documentation:   410.4

Analyte List*

Analyte Formula CAS Number Detection Limit
Chemical Oxygen Demand

* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.