Method 405.1 Water

Biochemical Oxygen Demand (5 Days, 20°C)

This test is for the determinatio of biological oxygen demand (BOD). from municipal and industrial wastewaters.  The BOD test is an empirical bioassay-type procedure which measures the dissolved oxygen consumed by microbial life while assimilating and oxidizing the organic matter present.  A waste sample is incubated for five days at  20°C in the dark.  Dissolved oxygen is measured before and after incubation using a modified Winkler or oxygen probe method (e.g., EPA Method 360.1 or 360.2).  The reduction in dissolved oxygen during the incubation period yields a measure of BOD.

Application of the test to organic waste discharges allows calculation of the effect of the discharges on the oxygen resources of the receiving water. Data from BOD tests are used for the development of engineering criteria for the design of wastewater treatment plants. The actual envrionmental conditions of temperature, biological population, water movement, sunlight, and oxygen concentration cannot be accurately reproduced in the laboratory.  Results must take into account the above factors when relating BOD results to stream oxygen demands.

(EPA: Office of Water)


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Method Data

Hold Times, Preservatives, Preps, Collection, Analytical & Documentation
Holding Time:   48 hours from sample collection to analysis.
Preservatives:   Cool sample to 4°C.
Required Preps:   1L HDPE bottle
Collection Method:   Grab sampling
Analytical Methodology:   Probe
Documentation:   405.1

Analyte List*

Analyte Formula CAS Number Detection Limit
Biological Oxygen Demand

* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.