This method describes the sample collection and analysis of enflurane and halothane. The sample is collected on two standard size charcoal tubes connected in series for each sample. Samples are desorbed with carbon disulfide and analyzed by gas chromatography (GC) using a flame ionization detector (FID).
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| Hold Times, Preservatives, Preps, Collection, Analytical & Documentation | |
|---|---|
| Holding Time: | Recovery remained above 86% for most samples after 15 days of storage @ 5°C and 25°C. |
| Preservatives: | Keep refrigerated or at ambient temperature. |
| Required Preps: | Solid sorbent tubes: Coconut shell charcoal tubes, 100mg/50mg (glass tubes 7-cm x 6-mm OD x 4-mm) . |
| Collection Method: | Solid sorbent charcoal tubes: 10L @ 0.1L/min. |
| Analytical Methodology: | GC-FID |
| Documentation: | 
029
|
| Analyte | Formula | CAS Number | Detection Limit | |
|---|---|---|---|---|
| Enflurane | C3H2ClF5O |
13838-16-9 |
3.04 |
µg |
| Halothane | C2HBrClF3 |
151-67-7 |
1.87 |
µg |
* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.