Method 29

Enflurane and Halothane


This method describes the sample collection and analysis of enflurane and halothane. The sample is collected on two standard size charcoal tubes connected in series for each sample. Samples are desorbed with carbon disulfide and analyzed by gas chromatography (GC) using a flame ionization detector (FID).


(OSHA)

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Method Data

Hold Times, Preservatives, Preps, Collection, Analytical & Documentation
Holding Time:   Recovery remained above 86% for most samples after 15 days of storage @ 5°C and 25°C.
Preservatives:   Keep refrigerated or at ambient temperature.
Required Preps:   Solid sorbent tubes: Coconut shell charcoal tubes, 100mg/50mg (glass tubes 7-cm x 6-mm OD x 4-mm) .
Collection Method:   Solid sorbent charcoal tubes: 10L @ 0.1L/min.
Analytical Methodology:   GC-FID
Documentation:   029

Analyte List*

Analyte Formula CAS Number Detection Limit
Enflurane
C3H2ClF5O
13838-16-9
3.04
 µg
Halothane
C2HBrClF3
151-67-7
1.87
 µg

* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.